ABSTRACT Objective: This study aimed to investigate the association between 25OHD (total, bioavailable and free) with bone mass and microarchitecture among primary hyperparathyroidism (PHPT) patients and controls. Subjects and methods: Sixty-four patients in the preoperative period of PHPT and 63 matched controls, who had not taken vitamin D in the last three months. To calculate the bioavailable and free 25OHD, the genetic variants of the vitamin D-binding protein (DBP) were determined. Bone mineral density (BMD) was determined by dual-energy X-ray absorptiometry (DXA). The distributions of total, bioavailable and free 25OHD and their correlation with TBS and DXA were evaluated. Results: PHPT showed BMD and TBS values lower than CTRL in all locations (p < 0.05). There were no statistical differences in the levels of free, bioavailable and total 25OHD between the PHPT and CTRL groups [mean, min-max: 3.4 (1.4-8.6) vs. 3.1 (1.0-9.8) pg/mL, 1.51 (0.43-3.58) vs. 1.41 (0.38-3.48) ng/mL, 22.6 (11.0-39.9) vs. 20.6 (8.9-35.3) ng/dL, respectively; (p > 0.05). The distribution of DBP haplotypes was similar between groups. DXA showed no correlation with any form of 25OHD in both groups. TBS presented a weak correlation with the total 25OHD in PHPT (r = 0.28; p = 0.02) and a moderate correlation with the total, free and bioavailable 25OHD in CTRL (r = 0.42; r = 0.42; r = 0.43; respectively, p < 0.01). Conclusion: The concentrations of total, free and bioavailable 25OHD were similar in both the PHPT and control groups. 25OHD concentrations correlated positively with TBS and not with DXA, especially in controls, suggesting that this method may be more sensitive to assessing the consequences of vitamin D deficiency on bone quality in individuals without PHPT.

ABSTRACT Objective To evaluate the usefulness of a third-generation PTH assay in the diagnosis of primary hyperparathyroidism (PHPT). Subjects and methods Forty-one PHPT patients (4 men and 37 women) with 61.2 ± 10.9 (mean ± SD) years, were studied and had PTH levels measured with two different methods using the same immunochemiluminescent assay plataform (Elecsys 2010 System, Roche). We compared a second-generation assay (I-PTH) with a third-generation PTH assay (Bio-PTH). Two populations of 423 and 120 healthy adults with serum 25OHD levels above 25 ng/mL were used to define normal values in the I-PTH and Bio-PTH assays respectively. Results Normal PTH values based in the healthy adults population were 24.2-78.0 pg/mL for the I-PTH assay and 19.9-58.5 pg/mL for Bio-PTH assay. In PHPT patients, PTH values ranged from 67 to 553 pg/mL (median: 168 pg/mL) using the I-PTH assay and from 55 to 328 pg/mL (median: 111 pg/mL) using the Bio-PTH assay. Results obtained with the Bio-PTH assay were significantly lower (p < 0.0001, Wilcoxon). In general I-PTH and Bio-PTH showed highly significant correlation (r = 0.952, p < 0.0001). Passing–Bablok analysis gave a regression equation of Bio PTH = 13.44 + 0.59 x intact PTH. PHPT patients had 25OHD levels ranging from 4 to 36 ng/mL (mean 16.2 ng/mL); 35 subjects (85.3%) had values bellow 25 ng/mL. Conclusion Our results demonstrate that both second and third generation PTH methods are strongly correlated in PHPT patients and control subjects. Lower results with Bio-PTH tests are expected in function of the assay specificity determined by the amino-terminal antibody used.

OBJETIVO: Avaliar o estado nutricional e o desenvolvimento neuropsicomotor em crianças freqüentadoras de uma creche. MÉTODOS: Estudo transversal de 113 crianças com seis a 70 meses de idade, que freqüentavam a creche Irmã Sheila em Catanduva, São Paulo. A avaliação nutricional foi feita por antropometria e analisada pela classificação de Waterlow e a avaliação do desenvolvimento neuropsicomotor pelo Teste de Denver II, em todas as crianças incluídas no estudo. Dados adicionais foram obtidos por meio de um questionário respondido pelos pais em 70% dos casos. RESULTADOS: Entre as crianças estudadas, observou-se 12% de desnutrição aguda, 1% de desnutrição pregressa e 16% de obesidade. O Teste de Denver II detectou 37% de suspeitos de atraso no desenvolvimento, sendo a linguagem a área mais acometida. A faixa etária mais comprometida foi a de 25 a 60 meses. O questionário mostrou que 87% das crianças receberam aleitamento materno (57% até o sexto mês), 96% das mães trabalhavam fora de casa, 54% delas possuíam ensino médio completo e 73% das famílias tinham renda máxima de dois salários-mínimos. CONCLUSÕES: A alta prevalência de distúrbios nutricionais e possíveis atrasos no desenvolvimento observados em crianças da creche Irmã Sheila mostraram a necessidade de introduzir dietas balanceadas, incentivando o desenvolvimento de hábitos alimentares saudáveis, além de alertar para a questão da interferência negativa dos fatores socioeconômicos e culturais no crescimento e desenvolvimento infantil.

OBJECTIVE: Evaluate the nutritional status and neurodevelopment of children enrolled in a day care center. METHODS: This cross-sectional study enrolled 113 children aged six to 70 months, assisted in a nonprofit day care center in Catanduva, São Paulo. All children were submitted to evaluation of the nutritional status by anthropometry and classified according to Waterlow criteria. Child development was assessed according to Denver II Test. Further characteristics were obtained by a questionnaire answered by 70% of the children's parents. RESULTS: Among the evaluated children, 12% had acute malnutrition, 1% past malnutrition and 16% obesity. The Denver II Test detected 37% of children with suspect of development delay (the language was the ability area more frequently affected). Children aged 25 to 60 months had more delays. Data from the questionnaire showed that 87% of children received breastfeeding (57% until around the sixth month of age), 96% of the mothers worked outside home, 54% of them had high school and 73% of the families received less than two minimum wages. CONCLUSIONS: The high prevalence of nutritional problems and possible developmental delays observed in this study indicates the need of adopting balanced diets and improving education towards healthier alimentary habits. Attention should be given in order to act on the negative influence of the poor socioeconomic and educational background on children's growth and development.